Quantitative evaluation of neurotrophin and trk mRNA expression in visual and limbic areas along the occipito‐temporo‐hippocampal pathway in adult macaque monkeys
Identifieur interne : 003912 ( Main/Exploration ); précédent : 003911; suivant : 003913Quantitative evaluation of neurotrophin and trk mRNA expression in visual and limbic areas along the occipito‐temporo‐hippocampal pathway in adult macaque monkeys
Auteurs : Hiroyuki Okuno [Japon] ; Wataru Tokuyama [Japon] ; Yue Xin Li [Japon] ; Takanori Hashimoto [Japon] ; Yasushi Miyashita [Japon]Source :
- Journal of Comparative Neurology [ 0021-9967 ] ; 1999-06-07.
English descriptors
- Teeft :
- Adult macaques, Anova, Antisense, Antisense trkb, Antisense trkc, Bdnf, Bdnf mrna, Brain areas, Cdna, Clone, Comp, Cortex, Cortical, Cortical areas, Cresyl, Cresyl violet, Crna, Crna probes, Cyclophilin, Cyclophilin gene, Cyclophilin mrna, Dentate, Dentate gyrus, Endogenous, Entorhinal, Entorhinal cortex, Error bars, Expression levels, Fragment, G6pd, Genbank, Gene, Granule, Granule cell layer, Gyrus, Hashimoto, Hilar region, Hippocampal, Hippocampal formation, Hippocampus, Hybridization, Hybridized, Image analyzer, Kinase, Limbic, Limbic areas, Lower panel, Macaque, Macaque monkey, Macaque monkeys, Medial, Miyashita, Mrna, Mrna expression, Mrna expression levels, Mrna quantification, Neocortical, Neural circuits, Neurol, Neuron, Neuronal, Neurosci, Neurotrophic, Neurotrophic factor, Neurotrophin, Neurotrophins, Northern blot analysis, Nucleotide sequences, Nucleotide substitutions, Okuno, Pathway, Perirhinal, Polymerase, Polymerase chain reaction, Present study, Primer, Primer pairs, Quantification, Quantitated, Receptor, Relative mrna levels, Same efficiency, Shelton, Standard gene, Sulcus, Synaptic, Target gene, Target genes, Trka, Trkb, Trkc, Tubulin, Upper panel, Visual areas.
Abstract
The neurotrophins have been implicated in shaping and remodeling the connectivity of neural circuits. To explore the role of neurotrophins and their receptors, Trks, in cortical neural circuits of adult macaque monkeys, we determined mRNA expression levels of neurotrophins and Trk receptors in various visual and limbic areas along the occipito‐temporo‐hippocampal pathway by using a quantitative reverse‐transcription polymerase chain reaction technique. The expression level of brain‐derived neurotrophic factor (BDNF) mRNA was lowest in the primary visual cortex (V1), moderate in the temporal visual association area, and highest in the hippocampus. The expression levels of trkB mRNA isoforms, the full‐length form that encodes a receptor tyrosine kinase and the truncated form that encodes a noncatalytic receptor, were also low in V1, moderate in the visual association area, and high in the entorhinal cortex. However, in contrast to their ligand BDNF, the expression levels of both trkB isoforms in the hippocampus were significantly lower than those in the entorhinal cortex. NT‐3 mRNA was detectable only in the hippocampus and the entorhinal cortex, whereas both the full‐length and the truncated forms of trkC mRNA were widely distributed throughout the neocortex and the limbic cortex. The expression levels of NGF and trkA mRNAs in these cortical areas were too low to determine quantitatively. The present findings suggest that, among neurotrophin/Trk signaling systems, the BDNF/TrkB‐mediated signal most likely contributes to stabilization, remodeling, or both, of neural circuits in cortical areas along the occipito‐temporo‐hippocampal pathway in the adult macaque monkey. J. Comp. Neurol. 408:378–398, 1999. © 1999 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/(SICI)1096-9861(19990607)408:3<378::AID-CNE6>3.0.CO;2-A
Affiliations:
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Kantō</region></placeName></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Mind Articulation Project, ICORP, JST, Yushima, Tokyo 113–0034</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>National Institute for Physiological Sciences, Myodaiji, Okazaki 444–8585</wicri:regionArea><wicri:noRegion>Okazaki 444–8585</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Comparative Neurology</title><idno type="ISSN">0021-9967</idno><idno type="eISSN">1096-9861</idno><imprint><biblScope unit="vol">408</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="378">378</biblScope><biblScope unit="page" to="398">398</biblScope><biblScope unit="page-count">21</biblScope><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><date type="published" when="1999-06-07">1999-06-07</date></imprint><idno type="ISSN">0021-9967</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-9967</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adult macaques</term><term>Anova</term><term>Antisense</term><term>Antisense trkb</term><term>Antisense trkc</term><term>Bdnf</term><term>Bdnf mrna</term><term>Brain areas</term><term>Cdna</term><term>Clone</term><term>Comp</term><term>Cortex</term><term>Cortical</term><term>Cortical areas</term><term>Cresyl</term><term>Cresyl violet</term><term>Crna</term><term>Crna probes</term><term>Cyclophilin</term><term>Cyclophilin gene</term><term>Cyclophilin mrna</term><term>Dentate</term><term>Dentate gyrus</term><term>Endogenous</term><term>Entorhinal</term><term>Entorhinal cortex</term><term>Error bars</term><term>Expression levels</term><term>Fragment</term><term>G6pd</term><term>Genbank</term><term>Gene</term><term>Granule</term><term>Granule cell layer</term><term>Gyrus</term><term>Hashimoto</term><term>Hilar region</term><term>Hippocampal</term><term>Hippocampal formation</term><term>Hippocampus</term><term>Hybridization</term><term>Hybridized</term><term>Image analyzer</term><term>Kinase</term><term>Limbic</term><term>Limbic areas</term><term>Lower panel</term><term>Macaque</term><term>Macaque monkey</term><term>Macaque monkeys</term><term>Medial</term><term>Miyashita</term><term>Mrna</term><term>Mrna expression</term><term>Mrna expression levels</term><term>Mrna quantification</term><term>Neocortical</term><term>Neural circuits</term><term>Neurol</term><term>Neuron</term><term>Neuronal</term><term>Neurosci</term><term>Neurotrophic</term><term>Neurotrophic factor</term><term>Neurotrophin</term><term>Neurotrophins</term><term>Northern blot analysis</term><term>Nucleotide sequences</term><term>Nucleotide substitutions</term><term>Okuno</term><term>Pathway</term><term>Perirhinal</term><term>Polymerase</term><term>Polymerase chain reaction</term><term>Present study</term><term>Primer</term><term>Primer pairs</term><term>Quantification</term><term>Quantitated</term><term>Receptor</term><term>Relative mrna levels</term><term>Same efficiency</term><term>Shelton</term><term>Standard gene</term><term>Sulcus</term><term>Synaptic</term><term>Target gene</term><term>Target genes</term><term>Trka</term><term>Trkb</term><term>Trkc</term><term>Tubulin</term><term>Upper panel</term><term>Visual areas</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The neurotrophins have been implicated in shaping and remodeling the connectivity of neural circuits. To explore the role of neurotrophins and their receptors, Trks, in cortical neural circuits of adult macaque monkeys, we determined mRNA expression levels of neurotrophins and Trk receptors in various visual and limbic areas along the occipito‐temporo‐hippocampal pathway by using a quantitative reverse‐transcription polymerase chain reaction technique. The expression level of brain‐derived neurotrophic factor (BDNF) mRNA was lowest in the primary visual cortex (V1), moderate in the temporal visual association area, and highest in the hippocampus. The expression levels of trkB mRNA isoforms, the full‐length form that encodes a receptor tyrosine kinase and the truncated form that encodes a noncatalytic receptor, were also low in V1, moderate in the visual association area, and high in the entorhinal cortex. However, in contrast to their ligand BDNF, the expression levels of both trkB isoforms in the hippocampus were significantly lower than those in the entorhinal cortex. NT‐3 mRNA was detectable only in the hippocampus and the entorhinal cortex, whereas both the full‐length and the truncated forms of trkC mRNA were widely distributed throughout the neocortex and the limbic cortex. The expression levels of NGF and trkA mRNAs in these cortical areas were too low to determine quantitatively. The present findings suggest that, among neurotrophin/Trk signaling systems, the BDNF/TrkB‐mediated signal most likely contributes to stabilization, remodeling, or both, of neural circuits in cortical areas along the occipito‐temporo‐hippocampal pathway in the adult macaque monkey. J. Comp. Neurol. 408:378–398, 1999. © 1999 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Okuno, Hiroyuki" sort="Okuno, Hiroyuki" uniqKey="Okuno H" first="Hiroyuki" last="Okuno">Hiroyuki Okuno</name></region><name sortKey="Hashimoto, Takanori" sort="Hashimoto, Takanori" uniqKey="Hashimoto T" first="Takanori" last="Hashimoto">Takanori Hashimoto</name><name sortKey="Li, Yue Xin" sort="Li, Yue Xin" uniqKey="Li Y" first="Yue Xin" last="Li">Yue Xin Li</name><name sortKey="Miyashita, Yasushi" sort="Miyashita, Yasushi" uniqKey="Miyashita Y" first="Yasushi" last="Miyashita">Yasushi Miyashita</name><name sortKey="Miyashita, Yasushi" sort="Miyashita, Yasushi" uniqKey="Miyashita Y" first="Yasushi" last="Miyashita">Yasushi Miyashita</name><name sortKey="Miyashita, Yasushi" sort="Miyashita, Yasushi" uniqKey="Miyashita Y" first="Yasushi" last="Miyashita">Yasushi Miyashita</name><name sortKey="Okuno, Hiroyuki" sort="Okuno, Hiroyuki" uniqKey="Okuno H" first="Hiroyuki" last="Okuno">Hiroyuki Okuno</name><name sortKey="Okuno, Hiroyuki" sort="Okuno, Hiroyuki" uniqKey="Okuno H" first="Hiroyuki" last="Okuno">Hiroyuki Okuno</name><name sortKey="Tokuyama, Wataru" sort="Tokuyama, Wataru" uniqKey="Tokuyama W" first="Wataru" last="Tokuyama">Wataru Tokuyama</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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